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Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Study Limitations
We only investigated the role of a single-nucleotide polymorphism (SNP) on physiologic function. Previous work by Drysdale and colleagues sequenced the entire P2AR in a large population of asthmatics. They subsequently grouped the SNPs according to haplotypes; and while no SNP was associated with response to a P-agonist, combinations of SNPs grouped according to haplotype […]
Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Conclusion
Interestingly, the difference between the Arg16 and Gly16 genotypes in FEF50 occurred during a time when catecholamines were rapidly returning to baseline after the airway P2ARs had been exposed to catecholamines for 10 to 15 min (exercise plus early cool down). This may suggest that significant in vivo desensitization takes between 9 min and 15 […]
Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Discussion
The coding region of the P2AR demonstrates multiple sites of polymorphic variation such as positions 16, 27, and 164. Positions 16 and 27 have been studied extensively both in vitro and in oioo. In vitro, the most impaired polymorphic receptor appears to be due to a threonine to isoleucine change at amino acid 164. However, […]
Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Airway Responses to Exercise
The focus of our study was to examine the influence of variation in the P2AR gene at codon 16 on the airway responses to exercise in young, healthy adults. We found that the degree of bronchodilation with light- and heavy-intensity exercise was similar between the homozygous Arg16 and Gly16 subjects. However, during the recovery period, […]
Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Catecholamine Response to Exercise
Figure 1 shows the changes in arterial levels of epinephrine and norepinephrine during low- and high-intensity exercise as well as during recovery (expressed as the percentage change from rest). No significant differences were observed between groups, although the Arg16 subjects tended to have a greater increase in epinephrine than the Gly16 subjects (p = 0.07). […]
Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Subject Characteristics
The demographic data were examined using independent t tests with an a = 0.05. We used analysis of variance to examine differences in the primary outcome measure, FEF50 (SPSS; Chicago, IL). An analysis of covariance was also performed between the groups with epinephrine as the covariate and FEF50 as the dependent variable. Prior to beginning […]
Influence of p2-Adrenergic Receptor Genotype on Airway Function During Exercise in Healthy Adults: Data Collection
Cardiopulmonary Assessment During Exercise: Measurements of oxygen uptake (Vo2) and the elimination of carbon dioxide were measured continuously during the various exercise tests and stages using a metabolic cart (Medical Graphics; St. Paul, MN) interfaced with a mass spectrometer (Perkin Elmer; Wellesley, MA). This system has been validated against classic “bag” collection techniques, and stability […]